Large-scale Biometrics Deployment in Europe: Identifying Challenges and Threats

With large-scale biometrics deployment in the EU still in its infancy and with stakeholders racing to position themselves in view of the lucrative market that is forecasted, a study to identify challenges and threats that need to be dealt with was launched. This is the result: a report on Biometrics large-scale Deployment in Europe. The report tackles three main issues namely, the status, security / privacy and testing / certification processes. A survey was launched so as to help reveal the actual status of Biometrics large-scale Deployment initiatives in EU. The main outcome of the survey was that an open dissemination of implementation results policy is needed mainly on deployment plans, strategies, barriers and best practices. The security/ privacy challenges study identified a number of issues, the most important of which were related to proportionality and compliance to the existing regulatory framework while at the same time it revealed an important number of related actions aiming at ensuring both data security and privacy. The aim of the Bio Testing Europe study was double: to identify and collect comparable and certified results under different technologies, vendors and environments situations and to feed in this information to animate discussion among the members of a European network which would enhance the European testing and certification capacity. The study presents an integrated picture of the identified issues as well as a number of recommendations. With some of the systems that are being implemented involving millions of individuals as target users it is important for policy makers to adopt some of the options presented so as to address the identified through the study challengesJRC.J.4-Information Societ

Rotating bending fatigue behaviour and quasi-static tensile properties of Wire Arc Additively Manufactured 308L stainless steel

Wire Arc Additive Manufacturing (WAAM) is a direct energy deposition method used to manufacture steel components by using an electric arc as a heat source to melt a metal wire and deposit it layer by layer. In this study, monotonic tensile tests, standardized Charpy impact tests, and rotating bending fatigue tests are executed to characterize the mechanical properties of WAAM 308L stainless steel using specimens extracted from additively manufactured plates. In particular, monotonic tensile properties are investigated in three directions: that is 0, 90, and 45 degrees with respect to the plane of deposition, whereas the fatigue strength is quantified for one direction only, i.e. 90 degrees since this is deemed to be the weakest.The mechanical characterization highlights that WAAM 308L SS shows an anisotropic behaviour, an enhanced strain-rate sensitivity, and an overall reduced yield strength as compared to the base material 308L. The anisotropic material behaviour is explained by the microstructure morphology since the austenite grains form anisotropic columnar zones due to an uneven heat profile during production. During the fatigue tests, the relatively high strain rate sensitivity causes susceptibility to self-heating at relatively low loading frequencies, i.e. below 100Hz

The high risk for type 2 diabetes among ethnic minority populations is not explained by low-grade inflammation

Our aim was to identify whether low-grade inflammation, reflected by C-reactive protein (CRP), explains the higher risk for incident type 2 diabetes (T2D) among ethnic minorities. We included 837 Dutch, 712 South-Asian Surinamese, 797 African Surinamese, 804 Ghanaian, 817 Turkish and 778 Moroccan origin participants of the HELIUS study (Amsterdam, the Netherlands). We used multiple linear regression to assess ethnic differences in CRP levels. We determined the association of CRP with T2D and the modifying effect of ethnicity by cox regression, and compared hazard ratios for the association between ethnicity and T2D before and after adjustment for CRP. CRP levels were higher in ethnic minority groups than in Dutch origin participants. CRP was associated with a higher T2D incidence, similarly across ethnic groups (overall HR per SD 1.38 [95% CI 1.14; 1.68]). However, the association was attenuated and no longer statistically significant after adjustment for adiposity measures (HR 1.11 [95% CI 0.90; 1.37]). CRP accounted for a very small part of the ethnic differences in T2D, but only in models unadjusted for adiposity. Low-grade inflammation does not substantially contribute to the higher risk of T2D among ethnic minority populations compared to the Dutch

The high risk for type 2 diabetes among ethnic minority populations is not explained by low-grade inflammation

Our aim was to identify whether low-grade inflammation, reflected by C-reactive protein (CRP), explains the higher risk for incident type 2 diabetes (T2D) among ethnic minorities. We included 837 Dutch, 712 South-Asian Surinamese, 797 African Surinamese, 804 Ghanaian, 817 Turkish and 778 Moroccan origin participants of the HELIUS study (Amsterdam, the Netherlands). We used multiple linear regression to assess ethnic differences in CRP levels. We determined the association of CRP with T2D and the modifying effect of ethnicity by cox regression, and compared hazard ratios for the association between ethnicity and T2D before and after adjustment for CRP. CRP levels were higher in ethnic minority groups than in Dutch origin participants. CRP was associated with a higher T2D incidence, similarly across ethnic groups (overall HR per SD 1.38 [95% CI 1.14; 1.68]). However, the association was attenuated and no longer statistically significant after adjustment for adiposity measures (HR 1.11 [95% CI 0.90; 1.37]). CRP accounted for a very small part of the ethnic differences in T2D, but only in models unadjusted for adiposity. Low-grade inflammation does not substantially contribute to the higher risk of T2D among ethnic minority populations compared to the Dutch

Human plasma IgG1 repertoires are simple, unique, and dynamic

Although humans can produce billions of IgG1 variants through recombination and hypermutation, the diversity of IgG1 clones circulating in human blood plasma has largely eluded direct characterization. Here, we combined several mass-spectrometry-based approaches to reveal that the circulating IgG1 repertoire in human plasma is dominated by a limited number of clones in healthy donors and septic patients. We observe that each individual donor exhibits a unique serological IgG1 repertoire, which remains stable over time but can adapt rapidly to changes in physiology. We introduce an integrative protein- and peptide-centric approach to obtain and validate a full sequence of an individual plasma IgG1 clone de novo. This IgG1 clone emerged at the onset of a septic episode and exhibited a high mutation rate (13%) compared with the closest matching germline DNA sequence, highlighting the importance of de novo sequencing at the protein level. A record of this paper's transparent peer review process is included in the supplemental information

Depicting the composition of gut microbiota in a population with varied ethnic origins but shared geography

Trillions of microorganisms inhabit the human gut and are regarded as potential key factors for health1,2. Characteristics such as diet, lifestyle, or genetics can shape the composition of the gut microbiota2–6 and are usually shared by individuals from comparable ethnic origin. So far, most studies assessing how ethnicity relates to the intestinal microbiota compared small groups living at separate geographical locations7–10. Using fecal 16S ribosomal RNA gene sequencing in 2,084 participants of the Healthy Life in an Urban Setting (HELIUS) study11,12, we show that individuals living in the same city tend to share similar gut microbiota characteristics with others of their ethnic background. Ethnicity contributed to explain the interindividual dissimilarities in gut microbiota composition, with three main poles primarily characterized by operational taxonomic units (OTUs) classified as Prevotella (Moroccans, Turks, Ghanaians), Bacteroides (African Surinamese, South-Asian Surinamese), and Clostridiales (Dutch). The Dutch exhibited the greatest gut microbiota α-diversity and the South-Asian Surinamese the smallest, with corresponding enrichment or depletion in numerous OTUs. Ethnic differences in α-diversity and interindividual dissimilarities were independent of metabolic health and only partly explained by ethnic-related characteristics including sociodemographic, lifestyle, or diet factors. Hence, the ethnic origin of individuals may be an important factor to consider in microbiome research and its potential future applications in ethnic-diverse societies

Human plasma IgG1 repertoires are simple, unique, and dynamic

Although humans can produce billions of IgG1 variants through recombination and hypermutation, the diversity of IgG1 clones circulating in human blood plasma has largely eluded direct characterization. Here, we combined several mass-spectrometry-based approaches to reveal that the circulating IgG1 repertoire in human plasma is dominated by a limited number of clones in healthy donors and septic patients. We observe that each individual donor exhibits a unique serological IgG1 repertoire, which remains stable over time but can adapt rapidly to changes in physiology. We introduce an integrative protein- and peptide-centric approach to obtain and validate a full sequence of an individual plasma IgG1 clone de novo. This IgG1 clone emerged at the onset of a septic episode and exhibited a high mutation rate (13%) compared with the closest matching germline DNA sequence, highlighting the importance of de novo sequencing at the protein level. A record of this paper's transparent peer review process is included in the supplemental information

Into the dark serum proteome: personalized features of IgG1 and IgA1 repertoires in severe COVID-19 patients

: Serum proteomics has matured and is now able to monitor hundreds of proteins quantitatively in large cohorts of patients. However, the fine characteristics of some of the most dominant proteins in serum, the immunoglobulins, are in these studies often ignored, due to their vast, and highly personalized, diversity in sequences. Here, we focus exclusively on these personalized features in the serum proteome, and distinctively chose to study individual samples from a low diversity population: elderly donors infected by SARS-CoV-2. By using mass spectrometry-based methods immunoglobulin IgG1 and IgA1 clonal repertoires were monitored quantitatively and longitudinally in more than 50 individual serum samples obtained from 17 COVID-19 patients admitted to intensive care units. These clonal profiles were used to examine how each patient reacted to a severe SARS-CoV-2 infection. All 17 donors revealed unique polyclonal repertoires and substantial changes over time, with several new clones appearing following the infection, in a few cases leading to a few, very high, abundant clones dominating their repertoire. Several of these clones were de novo sequenced through combinations of top-down, middle-down and bottom-up proteomics approaches. This revealed sequence features in line with sequences deposited in the SARS-CoV-specific antibody database. In other patients, the serological Ig profiles revealed the treatment with tocilizumab, that subsequently dominated their serological IgG1 repertoire. Tocilizumab clearance could be monitored and a half-life of approximately 6 days was established. Overall, our longitudinal monitoring of IgG1 and IgA1 repertoires of individual donors reveals that antibody responses are highly personalized traits of each patient, affected by the disease and the chosen clinical treatment. The impact of these observations argues for a more personalized and longitudinal approach in patients' diagnostics, both in serum proteomics as well as in monitoring immune responses